对 mRNA
poly(A) 的长度进行测量
主要测序方法包括:
PAL-seq
(2014; NGS; http://www.nature.com/doifinder/10.1038/nature13007)
TAIL-seq
(2014; NGS; http://dx.doi.org/10.1016/j.molcel.2014.02.007)
mTAIL-seq(2016; NGS; http://www.genesdev.org/cgi/doi/10.1101/gad.284802.116)
FLAM-seq
(2019, PacBio; https://www.nature.com/articles/s41592-019-0503-y)
PAIso-seq(2019; PacBio; https://www.nature.com/articles/s41467-019-13228-9)