按照教程进行运行的时候报错:
运行命令:
cluster0_conserved_markers <- FindConservedMarkers(seurat_integrated,
ident.1 = 0,
grouping.var = "sample",
only.pos = TRUE,
logfc.threshold = 0.25)
Error: Please install the metap package to use FindConservedMarkers.
This can be accomplished with the following commands:
----------------------------------------
install. Packages('BiocManager')
BiocManager::install('multtest')
install. Packages('metap')
----------------------------------------
即便安装完提示的所有包以后还是不行,参考seurat包的作者提供的操作安装额外的qqconf包也无法使用,检索以后发现有人提供的命令解决这问题:
packageurl <- "https://cran.r-project.org/src/contrib/Archive/metap/metap_1.0.tar.gz"
install.packages(packageurl, repos=NULL, type="source")
运行完以后FindConservedMarker命令可以进行