partition the cells into 14 clusters. These clusters could be assigned to nine known cell lineages through marker genes.
胃癌及其癌旁组织样本的单细胞类群鉴定：
- epithelium
(marked with EPCAM, KRT18 and KRT8), - parietal cell (marked with ATP4A),
- endocrine cell (marked with CHGA);
- B cells (marked with MS4A1 and CD79A);
- T cells (marked with CD2, CD3D and CD3E);
- macrophages
(marked with CD14); - mast cells (marked with CPA3);
- fibroblasts (marked with ACTA2 and COL1A2);
- endotheliocyte (marked with ENG and VWF).
非恶性上皮细胞亚群marker
- Surface cells (TFF2, GKN1 and MUC5AC)
- Chief cells ( PGA3, PGA4 and LIPF)
- SPEM : hyper- plastic/hypertrophic mucous neck cells (PGA3 and MUC6)
- Neck cell (MUC6, TFF2, CD44 and SOX9 and major histocompatibility complex class II (MHC-II) genes (eg, HLA-DPA1 and HLA-DRA))
基于细胞分化相关的marker,
- KRT20 and its positive-correlated genes (ie, PHGR1,MDK,CHDR2, RARRES3,GPA33,SLC5A134 and MUC13)
ref: Dissecting transcriptional heterogeneity in primary gastric adenocarcinoma by single cell RNA sequencing
未完待续 …………
