Schematic of a typical single-cell RNA-seq analysis workflow Ref: https://doi.org/10.15...
Schematic of a typical single-cell RNA-seq analysis workflow Ref: https://doi.org/10.15...
Ref:https://doi.org/10.1016/j.envpol.2023.121178[https://doi.org/10.1016/j.envpol.2023....
重叠延伸PCR技术 (gene splicing by overlap extension PCR,SOE PCR),是采用具有互补末端的引物,使 PCR 产物形成了重叠链,...
VLCFAs: very long chain fatty acidsVLCPUFAs: very long chain polyunsaturated fatty acid...
宏基因组下游分析 参考1: MicrobiotaProcess: A comprehensive R package for deep mining microbiome[h...
1.冷冻干燥:将成年斑马鱼麻醉(饥饿12h,排清粪便),放入1.5 mL或2 mL EP管中,液氮速冻后在-80 ℃冷冻 24 h,使用冻干机(Alpha 2-4 LSC)冷...
1. 组织固定根据所取斑马鱼组织大小,加入20倍体积的4%多聚甲醛(PFA)固定液,4℃固定2 days。 2. 脱水将组织使用乙醇进行梯度脱水-30% Ethanol @...
|软件名| 参考文献| FastQC | https://www.bioinformatics.babraham.ac.uk/projects/fastqc/[https:...
通过三个实验模型进行功能研究1.OxLDL(氧化的低密度脂蛋白) 暴露的泡沫细胞和高脂饮食 (HFD) 喂养的斑马鱼幼鱼模型:-PHLPP1 水平均升高;-通过过度表达和敲低...