求解答：输入python figaro.py -i /media/a/FA6C7F276C7EDE37/16s/data -o /media/a/FA6C7F276C7EDE37/16s/data -f 1 -r 1 -a 465 -F zymo
出现报错：Traceback (most recent call last):
File "figaro.py", line 213, in <module>
resultTable, forwardCurve, reverseCurve = figaroSupport.trimParameterPrediction.performAnalysisLite(parameters.inputDirectory.value, parameters.minimumCombinedReadLength.value, subsample = parameters.subsample.value, percentile = parameters.percentile.value, forwardPrimerLength=parameters.forwardPrimerLength.value, reversePrimerLength=parameters.reversePrimerLength.value, namingStandardAlias=fileNamingStandard)
File "/home/a/figaro/figaroSupport/trimParameterPrediction.py", line 436, in performAnalysisLite
forwardReadLength, reverseReadLength = checkReadLengths(fastqList)
File "/home/a/figaro/figaroSupport/trimParameterPrediction.py", line 398, in checkReadLengths
raise fastqHandler.FastqValidationError("Unable to validate fastq files enough to perform this operation. Please check log for specific error(s).")
figaroSupport.fastqHandler.FastqValidationError: Unable to validate fastq files enough to perform this operation. Please check log for specific error(s).
看了log文件是空白的，请问这是fastq文件内容有问题么?
微生物多样性qiime2分析流程(3) 使用figaro来得到dada2正反向截断参数dada2插件对原始数据paired-end-demux.qza进行质量过滤需要两个参数，trunc-len-f和trunc-len-r。这个想法是通过尽可能多地删除较低质量...