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  • 请问老师构建数据库的时候报错如何解决?我输入的命令是kraken2-build --standard --threads 24 --db $DBNAME
    Downloading nucleotide gb accession to taxon map...rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::10): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::13): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (130.14.250.7): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (165.112.9.229): Network is unreachable (101)
    rsync error: error in socket IO (code 10) at clientserver.c(125) [Receiver=3.1.2]

    Kraken2安装与数据库构建2022-01-02

    Kraken2是一款快速宏基因组DNA序列进行物种注释的软件。我将其用于测序数据以及序列的物种的物种注释,检查测序数据以及序列是否有污染。conda以及github网址:ht...

  • 请问构建数据库的时候报错如何解决?
    Downloading nucleotide gb accession to taxon map...rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::10): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (2607:f220:41e:250::13): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (130.14.250.7): Network is unreachable (101)
    rsync: failed to connect to ftp.ncbi.nlm.nih.gov (165.112.9.229): Network is unreachable (101)
    rsync error: error in socket IO (code 10) at clientserver.c(125) [Receiver=3.1.2]

    如何构建kraken2个性化数据库

    kraken2就不介绍了,是一款挺好用的快速比对宏基因组软件。 现在有了nt数据库下面animal的序列,那么如何构建kraken2的数据库呢? 首先推荐把kraken2安装...

  • ~/software/Bracken/bracken-build -d ~/db/minikraken2_v1_8GB -t 8 -k 35 -l 150
    这个命令会报错ERROR: Database library ~/kraken2/minikraken2_v1_8GB/library does not exist
    请问怎么解决呀?

    Kraken2+Bracken

    1. 简介 Kraken2是一个基于k-mer算法的高精度宏基因组序列分类软件,能够快速的将测序reads进行物种分类。 Kraken2官网[http://ccb.jhu.e...