衣锦夜行

Process Process-9:
Traceback (most recent call last):
File "/home/lihaoxing/miniconda3/envs/jupyter/lib/python3.9/site-packages/multiprocess/process.py", line 315, in _bootstrap
self.run()
File "/home/lihaoxing/miniconda3/envs/jupyter/lib/python3.9/site-packages/multiprocess/process.py", line 108, in run
self._target(*self._args, **self._kwargs)
File "/home/lihaoxing/miniconda3/envs/jupyter/lib/python3.9/site-packages/flask/app.py", line 1188, in run
run_simple(t.cast(str, host), port, self, **options)
File "/home/lihaoxing/miniconda3/envs/jupyter/lib/python3.9/site-packages/werkzeug/serving.py", line 1069, in run_simple
fd = int(os.environ["WERKZEUG_SERVER_FD"])
File "/home/lihaoxing/miniconda3/envs/jupyter/lib/python3.9/os.py", line 679, in __getitem__
raise KeyError(key) from None
KeyError: 'WERKZEUG_SERVER_FD'
​
请问有没有遇到这个问题，应该怎么解决

数据分析实战 | Jupyter notebook + Higlass 实现 Hi-C数据可视化

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衣锦夜行

您好，请问一下基于STAR分析后，有没有什么gff基因注释准确性高的软件，看您结尾说“Genemark-et结果一定会有很高的假阳性”，这可能不是理想的结果，因为就是因为基因组原始的gff不够准确，所以打算用RNAseq重新注释一遍😂

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xuzhougeng

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